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Separation of Pigments from the Extract of Spinach Leaves by Paper Chromatography

Paper chromatography is an analytical method, used to separate coloured substances, especially pigments. In paper chromatography, the sample is distributed between a stationary phase and a mobile phase. The stationary phase is usually a piece of high quality filter paper. The mobile phase is a developing solution that travels up the stationary phase, carrying the samples with it. When mobile phase is moved over the sample on the stationary phase, the components of the sample gets gradually separated from one another.


This video explains how to separate the pigments from the extract of spinach leaves by paper chromatography.


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